| Annotated protein: | Delphilin (Glutamate receptor, ionotropic, delta 2-interacting protein 1). Gene symbol: GRID2IP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q0QWG9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRID2IP |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | cerebellum, glutamatergic Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Miyagi Y, et al. "Delphilin: a novel PDZ and formin homology domain-containing protein that synaptically colocalizes and interacts with glutamate receptor delta 2 subunit" J Neurosci. 2002 Feb 1;22(3):803-14 PMID:11826110 |
| Figure(s): | Fig. 9 a,b,e,f |
| Annotation description: | Figure 9 shows the ultrastructural localization of Delphilin (A-C, arrowheads) at Purkinje cell synapses. Fig. 9A): Delphilin labelling at parallel fibre synapses. Purkinje cell spines forming synaptic contact with parallel fibre terminals (PF). The quantitative analysis revealed a normal distribution of gold particles from presynapse to postsynapse, with a peak at 8-16 nm postsynaptic from the midline of the synaptic cleft (Fig. 9E). The postsynaptic membrane outside the PSD was associated with very low immunogold labelling (Fig. 9F). 18/01/2021 Dnyanada: The paper provides no information on the validation/specificity of the anti-Delphilin antibody used in the study. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4510 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |