| Annotated protein: | Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Brain calcium channel I) (BI) (Calcium channel, L type, alpha-1 polypeptide, isoform 4) (Rat brain class A) (RBA-I) (Voltage-gated calcium channel subunit alpha Cav2.1). Gene symbol: CACNA1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P54282 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Nakamura Y, et al. "Nanoscale distribution of presynaptic Ca(2+) channels and its impact on vesicular release during development" Neuron. 2015 Jan 7;85(1):145-158 PMID:25533484 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1A: SDS-digested freeze-fracture replica labelling (SDS-FRL) with an antibody specific for the Cacna1a subunit of the Cav2.1 P/Q-type VGCCs shows immunogold particles (5 nm diameter) for Cav2.1 are predominantly found on the presynaptic P-face, with little labelling on the exoplasmic face or cross-fractured face. Figure S1A-B: Specificity of the antibody in replica labelling was confirmed using Cav2.1 knockout mice which showed little Cav2.1 labelling. Figure S2C-D: Cav2.1 labelling followed by RIM staining revealed that the majority of Cav2.1 clusters are closely associated with RIM particles supporting that Cav2.1 are localized in AZs. The term "integral component.." was chosen based on the transmembrane topology of the Cacna1a subunit (P54282_RAT) Note: Reference tracking shows that the antibody used in the study is specific against the Cacna1a subunit of the Cav2.1 channel (PMID: 22279216). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4541 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |