| Annotated protein: | Neuritin. Gene symbol: NRN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O08957 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic endocytic zone (GO:0098843) |
| Annotated paper: | Cottrell JR, et al. "CPG2: a brain- and synapse-specific protein that regulates the endocytosis of glutamate receptors" Neuron. 2004 Nov 18;44(4):677-90 PMID:15541315 |
| Figure(s): | Figure 2, 3, 4 |
| Annotation description: | Figure 2. Immunostaining and confocal imaging of hippocampal rat neurons show Neuritin (NRN1/CPG2) is localized to the postsynapse of glutamatergic neurons. Figure 3. High-magnification images show that within the dendritic spines, Neuritin is localized just beside the PSD. Similar observations were made in subsequent immunoEM experiments (Fig. 4c-e). Figure 4. cultured hippocampal rat neurons infected with a lentivirus expressing a clathrin light chain A1-GFP fusion protein (green) and stained for CPG2 (red) and PSD-95 (blue) showed co-localization of Neuritin (CPG2) and Clathrin light chain A1 (CLTA) in the postsynapse. Similar observations were made in subsequent immunoEM experiments (Fig. 3b-c). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4646 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |