| Annotated protein: | Inhibitory synaptic factor 1 (InSyn1). Gene symbol: INSYN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CD60 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ INSYN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization (GO:0099572) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Uezu A, et al. "Identification of an elaborate complex mediating postsynaptic inhibition" Science. 2016 Sep 9;353(6304):1123-9 PMID:27609886 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3a,b: HA-tagged InSyn1 was coexpressed in primary neuronal cells from mouse hippocampus with GPHN.FingR-GFP, a recombinant protein sensor of endogenous gephyrin (GPHN, a marker for inhibitory synapses) fused to green fluorescent protein (GFP). co-localization was ~60%. Figure 3c: InSyn1 coimmunoprecipitates with gephyrin (GPHN) when coexpressed in human embryonic kidney 293 (HEK293) cells. |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 1B: InSyn1 and Gephyrin co-localize (reduced upon α-dystroglycan depletion) @ PMID:31829939 |
| SynGO annotation ID: | 4810 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |