| Annotated protein: | Ras-related protein Rab-7a (EC 3.6.5.2) (Ras-related protein BRL-RAS) (Ras-related protein p23). Gene symbol: RAB7A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P09527 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAB7A |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle recycling via endosome (GO:0036466) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Truckenbrodt S, et al. "Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission" EMBO J. 2018 Aug 1;37(15):e98044 PMID:29950309 |
| Figure(s): | Figure S30 |
| Annotation description: | Rab7 is a marker for late endosomes (Zerial and McBride, 2001. PMID:11252952) "The inactivated synaptic vesicles must ultimately be degraded at a later time point. Synaptic vesicle degradation is widely assumed to entail fusion to the endo-lysosomal system (Katzmann et al, 2002; Raiborg & Stenmark, 2009; Rizzoli, 2014). To test whether the inactivated vesicles are indeed targeted for degradation, we tested their co-localization with the recycling endosome marker Rab7, which is thought to be the first step in vesicle degradation (Rizzoli, 2014). We took advantage of experiments in which SNAP25 or sypHy-SNAP25 was overexpressed, and found that the vesicles in both cases co-localized more strongly with Rab7 than control vesicles (Appendix Fig S30)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Rab7 is a marker for late endosomes @ PMID:11252952 |
| SynGO annotation ID: | 4850 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |