| Annotated protein: | Dystrobrevin beta (DTN-B) (mDTN-BDTN-B) (Beta-dystrobrevin). Gene symbol: DTNB. Taxonomy: Mus musculus (Mouse). Uniprot ID: O70585 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DTNB |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Fujimoto T, et al. "Generation of dystrophin short product-specific tag-insertion mouse: distinct Dp71 glycoprotein complexes at inhibitory postsynapse and glia limitans" Cell Mol Life Sci. 2022 Jan 30;79(2):109 PMID:35098363 |
| Figure(s): | 6d,g |
| Annotation description: | Figure 6d,g: confocal imaging and double immunostaining showed dystrobrevin beta (DTNB) and Gephyrin (GPHN), an inhibitory postsynapse marker, co-localized in the hippocampal dentate gyrus of mouse brain sections. Authors note that "Dtnb was co-labelled with Gephyrin (Fig. 6g) but not with PSD95 (Fig. 6h), indicating that Dtnb was exclusively present at inhibitory postsynapses." Furthermore, DTNB was identified as a binding partner of Dp71 (short product of Dystrophin, DMD, which is also shown to be present in inhibitory synapses in this paper) by IP and both proteins seemed to co-localize at dendritic puncta (Fig. 6). The gephyrin staining had cross reactivity with the nucleus in Figures 3 and 4, on which authors noted: "Arrows indicate strong cross-reactions against nuclear component revealed by anti-Gephyrin antibody which should be ignored". This brings the specificity of the gephyrin antibody into question. Additional annotations of independent experimental evidence would be helpful to support these observations. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4939 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |