| Annotated protein: | Serine/threonine-protein kinase PAK 2 (EC 2.7.11.1) (Gamma-PAK) (p21-activated kinase 2) (PAK-2) [Cleaved into: PAK-2p27; PAK-2p34]. Gene symbol: PAK2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CIN4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PAK2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | forebrain, glutamatergic |
| Annotated paper: | Hayashi ML, et al. "Altered cortical synaptic morphology and impaired memory consolidation in forebrain- specific dominant-negative PAK transgenic mice" Neuron. 2004 Jun 10;42(5):773-87 PMID:15182717 |
| Figure(s): | Fig. 1 |
| Annotation description: | "Biochemical fractionation followed by Western blot analysis reveals the presence of active PAK in the PSD fraction. Phosphorylated forms of PAK1, PAK2, and PAK3 (p-PAK1/2/3) were all present in the PSD fraction, while un-phosphorylated PAK1/3 (Up-PAK1/3) were nearly absent from the PSD fraction. As detected by the p-PAK antibody, the amount of p-PAK1 was much higher than that of p-PAK2 and p-PAK3. 29/8/2017 Pim - Authors state: "p-PAK predominantly localizes in the cell soma, dendrites, and dendritic spines while it is largely excluded from the axons and boutons of mature cortical neurons" based on IF data. However this is with a pan-PAK antibody and cannot be used w/o the biochemical purification data. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| SynGO annotation ID: | 501 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |