| Annotated protein: | Connector enhancer of kinase suppressor of ras 2 (Connector enhancer of KSR 2) (CNK homolog protein 2) (CNK2) (Membrane-associated guanylate kinase-interacting protein) (Maguin). Gene symbol: CNKSR2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z1T4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNKSR2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic specialization organization (GO:0099084) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zieger HL, et al. "Disease-associated synaptic scaffold protein CNK2 modulates PSD size and influences localisation of the regulatory kinase TNIK" Sci Rep. 2020 Mar 31;10(1):5709 PMID:32235845 |
| Figure(s): | Figures 1-6 |
| Annotation description: | CNK2 (gene CNKSR2) is localized at/near the PSD (Fig. 1), loss of CNK2 reduced PSD size (Fig. 2) and expression of a CNK2 variant that does not bind to the membrane affected PSD morphology (Fig. 3). CNK2 is attached to phospholipid membranes via its PH domain (Fig. S2), interacts with TNIK (Fig. 4,5) and EGFP-CNK2ΔPH reduced localization of TNIK to the postsynapse. |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5094 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |