| Annotated protein: | Dihydropyrimidinase-related protein 2 (DRP-2) (Collapsin response mediator protein 2) (CRMP-2) (Turned on after division 64 kDa protein) (TOAD-64). Gene symbol: DPYSL2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P47942 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DPYSL2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | dorsal root ganglion (DRG) |
| Annotated paper: | Chi XX, et al. "Regulation of N-type voltage-gated calcium channels (Cav2.2) and transmitter release by collapsin response mediator protein-2 (CRMP-2) in sensory neurons" J Cell Sci. 2009 Dec 1;122(Pt 23):4351-62 PMID:19903690 |
| Figure(s): | Figure 6 |
| Annotation description: | "Double-labeling of sensory neurons with antibodies against CRMP-2 and the synaptic vesicle marker synaptophysin (Ichikawa et al., 1991) revealed extensive colocalization of CRMP-2 in synaptophysin-positive puncta (Fig. 6B), wherein 85±12% (n=468) of synapses were labeled with both proteins (Fig. 6D). To test whether endogenous CRMP-2 was targeted to functionally active synapses and/or release sites, neurons were loaded with the membrane-bound dye FM4-64 by stimulation with 90 mM KCl. Neurons loaded with FM dye were then fixed and labeled with an antibody against CRMP-2 (Fig. 6Ci). Of the functionally active FM-dye-loaded release sites, 78±17% (n=347) were positive for CRMP-2, thus indicating that CRMP-2 is targeted to functional synapses (Fig. 6Ciii,D)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | CRMP2 interacts with CaV2.2, is localized in synaptic biochemical fractions and its overexpression increased presynaptic bouton size @ PMID:19755421 |
| SynGO annotation ID: | 5121 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |