| Annotated protein: | Neurabin-2 (Neurabin-II) (Neural tissue-specific F-actin-binding protein II) (PP1bp134) (Protein phosphatase 1 regulatory subunit 9B) (Spinophilin) (p130). Gene symbol: PPP1R9B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O35274 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP1R9B |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic membrane (GO:0098890) |
| Synapse type(s): | hippocampus, glutamatergic hypothalamus, glutamatergic |
| Annotated paper: | Allen PB, et al. "Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines" Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9956-61 PMID:9275233 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3: "RU144 (Fig. 3) and RU145 (not shown) were used to examine the distribution of the protein in rat brain by immunohistochemistry. The two antibodies gave identical staining patterns. At the light microscopic level, immunoreactivity appeared as very numerous small puncta, 0.5-1 μm in diameter (Fig. 3C). These immunoreactive puncta were abundant in the neuropil of virtually all brain regions examined. In the areas examined by electron microscopy (hippocampus and caudatoputamen), these puncta were resolved as dendritic spines (Fig. D and E). Immunoreactivity was intense in the spine neck and especially in the spine head, and disappeared abruptly at the junction between the spine neck and dendritic shaft (Fig. 3E). Within dendritic spines, immunoreaction product adhered to the cytoplasmic surface of the plasmalemma, the spine apparatus, and the postsynaptic density. Light microscopy showed that the intensity of dendritic spine immunoreactivity varied from region to region. The strongest immunoreactivity was present in the hippocampal formation and moderate levels were seen in caudatoputamen and dorsal thalamus (Fig. 3A)." "Immunoreactivity is not present in axon terminals (AT) or in the dendritic shaft (Den)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Neurabin-II/spinophilin is an actin filament-binding protein with one pdz domain. Neurabin-II/spinophilin was enriched at the postsynaptic density fraction in rat brain. Immunofluorescence microscopic analysis revealed that neurabin-II was highly concentrated at the synapse in primary cultured rat hippocampal neurons and at the cadherin-based cell-cell adhesion sites in Madin-Darby canine kidney cells. @ PMID:9452470 |
| SynGO annotation ID: | 5190 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |