Annotated protein:Dedicator of cytokinesis protein 1 (180 kDa protein downstream of CRK) (DOCK180). Gene symbol: DOCK1. Taxonomy: Homo sapiens (Human). Uniprot ID: Q14185
antibody wiki:
SynGO gene info:SynGO data @ DOCK1
Ontology domain:Biological Process
SynGO term:regulation of postsynapse assembly (GO:0150052)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Kim JY, et al. "The RhoG/ELMO1/Dock180 signaling module is required for spine morphogenesis in hippocampal neurons" J Biol Chem. 2011 Oct 28;286(43):37615-24 PMID:21900250
Figure(s):Figure 1, 3, 4
Annotation description:Figure 1b, 3e: immunofluorescence imaging of hippocampal neurons stained for endogenous Dock180 (DOCK1) and PSD95 (DLG4) showed partial co-localization. Stainings of Dock180 were apposed to presynaptic synapsin-1 (SYN1). Overexpressed FLAG-Dock180 localized to dendrites and spines.

Figure 4: ELMO and Dock180 (DOCK1) form a complex in hippocampal neurons and function through activating Rac1. Overexpressed Dock180 increased spinogenesis.

Figure 5-7: RhoG is required for spine formation, its activation leads to increased spine head size and functions through the ELMO1/Dock180/Rac pathway.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Antibody (detection)
RNAi / shRNA
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5208
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology