| Annotated protein: | Nck-associated protein 1 (NAP 1) (Membrane-associated protein HEM-2) (p125Nap1). Gene symbol: NCKAP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P55161 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NCKAP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chazeau A, et al. "Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion" EMBO J. 2014 Dec 1;33(23):2745-64 PMID:25293574 |
| Figure(s): | Figure 3, S3 |
| Annotation description: | Figure 1: overexpression of mEOS2-Nap1 (Nck-associated protein 1, part of the WAVE complex, gene NCKAP1) in dissociated hippocampal neurons followed by super-resolution imaging showed localization to dendritic spine heads. "Live super-resolution intensity images and dual-color PALM/dSTORM experiments demonstrated that mEOS2-Nap1, like mEOS2-Abi1, was not localized at protruding tips, but also clustered into a single central domain having the same size and overlapping with PSD-95 (Fig 3D and E, Supplementary Figs S3 and S5)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5374 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |