| Annotated protein: | Neuroligin-1. Gene symbol: NLGN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99K10 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NLGN1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic density assembly (GO:0099151) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Letellier M, et al. "Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation" Elife. 2020 Apr 23;9:e52027 PMID:32324534 |
| Figure(s): | Figure 2, 3, 4 |
| Annotation description: | Figure 2b: immunofluorescence confocal imaging of overexpressed HA-tagged optoFGFR1 (photoactivatable version of FGFR1) in mouse hippocampal neurons showed localization to dendritic shafts and dendritic spines. Figure 2c: comparing between hippocampal cultures from wildtype and neuroligin-1 (NLGN1) mice, optogenetic stimulation of optoFGFR1 increases dendritic spine density in a Nlgn1-dependent manner. Figure 3, 4: Light activation of optoFGFR1 in CA1 neurons selectively increases AMPA receptor-mediated EPSCs in a Nlgn1-dependent manner. The light-induced increase in AMPA receptor-mediated EPSCs is specific to Y782 phosphorylation in Nlgn1. authors conclude: "Combined with computer simulations, our data support a model by which Nlgn1 tyrosine phosphorylation promotes the assembly of an excitatory post-synaptic scaffold that captures surface AMPA receptors. This optogenetic strategy highlights the impact of Nlgn1 intracellular signaling in synaptic differentiation and potentiation, while enabling an acute control of these mechanisms." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5420 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |