| Annotated protein: | Angiomotin. Gene symbol: AMOT. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A0G2JX94 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AMOT |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic actin cytoskeleton (GO:1905274) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Wigerius M, et al. "The polarity protein Angiomotin p130 controls dendritic spine maturation" J Cell Biol. 2018 Feb 5;217(2):715-730 PMID:29317530 |
| Figure(s): | Fig. 4; Fig. 3 |
| Annotation description: | AMOT-130 stabilizes the actin cytoskeleton in dendritic spines as shown by FRAP assay (Fig. 4). Mutations in AMOT (e.g. AMOT lacking the C-terminus) do not stabilize. Knock-down of AMOT with two different siRNAs destabilize PSD scaffolds and triggers spine elongation (Fig. 3). RNAi-resistent AMOT rescues. The process is regulated by phosphorylation of AMOT at S175 by Lats1 kinase, which removes AMOT from the synapse or prevents its entry. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Fluorescence recovery after photobleaching (FRAP) |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 5527 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |