| Annotated protein: | Protein kinase C beta type (PKC-B) (PKC-beta) (EC 2.7.11.13). Gene symbol: PRKCB. Taxonomy: Mus musculus (Mouse). Uniprot ID: P68404 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRKCB |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Fioravante D, et al. "Calcium-dependent isoforms of protein kinase C mediate posttetanic potentiation at the calyx of Held" Neuron. 2011 Jun 9;70(5):1005-19 PMID:21658591 |
| Figure(s): | Figure 1 |
| Annotation description: | Using a PKC beta specific antibody and knockout control, it is demonstrated that PKC beta is expressed at the presynaptic terminal of the calyx of held. 10/3/2017 Pim: -Literal: "In slices from wild-type mice, antibody labeling for PKCα and PKCβ overlapped with vGlut1 labeling, consistent with presynaptic localization of these kinases at the calyx of Held synapse." -PCKalpha has C2 domain and is able to interact with lipids membranes. In previous annotations of this expert it was established that only a small fraction of the total PKC pool is membrane attached; the rest resides in the cytoplasm. Therefore, the "cytosol" component of the term is inferred. Because domain information is not reflected in GO, I agreed to this term. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 69 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |