| Annotated protein: | Regulating synaptic membrane exocytosis protein 1 (Rab-3-interacting molecule 1) (RIM 1). Gene symbol: RIMS1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JIR4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMS1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone cytoplasmic component (GO:0098831) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tang AH, et al. "A trans-synaptic nanocolumn aligns neurotransmitter release to receptors" Nature. 2016 Aug 11;536(7615):210-4 PMID:27462810 |
| Figure(s): | Figure 1, 2 |
| Annotation description: | Using STORM super resolution microscopy and well-validated antibodies, it is demonstrated that RIM1/2 is localized at the active zone, directly opposed to PSD95. RIM1/2 localization is very similar to other well-validated active zone components, including Munc13 and Bassoon (Figure 1). RIM1/2 localization is also shown to correlate with the location of vesicle fusion (Figure 2). 23/01/2017 Pim - The RIM antibody (anti-RIM1/2 (1:500; Synaptic Systems No. 140203) used in Fig. 1 has a non-selective RIM1/RIM2 specificity. - The authors mention identical results with a RIM1 specific antibody ("Similar distribution and nanocluster properties were found using a different antibody targeted towards a separate epitope in RIM1 (Extended Data Fig. 2d)"). - presynaptic localization of overexpressed RIM1 is shown in Fig.2 ("To assess these predictions, we mapped vesicle fusion sites relative to Eos3-tagged RIM1 using sequential PALM-pHuse imaging on the same live boutons (Fig. 2h, Extended Data Fig. 6d, e)") - Overall these data justify annotation against RIM1. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Shows specificity of the antibody used in this publication with a KO control. @ PMID:27537483 |
| SynGO annotation ID: | 72 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |