Annotated protein:	Sodium- and chloride-dependent GABA transporter 1 (GAT-1) (Solute carrier family 6 member 1). Gene symbol: SLC6A1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P31648
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SynGO gene info:	SynGO data @ SLC6A1
Ontology domain:	Biological Process
SynGO term:	neurotransmitter reuptake (GO:0098810)
Synapse type(s):	hippocampus, GABAergic
Annotated paper:	Jensen K, et al. "GABA transporter-1 (GAT1)-deficient mice: differential tonic activation of GABAA versus GABAB receptors in the hippocampus" J Neurophysiol. 2003 Oct;90(4):2690-701 PMID:12815026
Figure(s):	Figue 1D, 2, 4, 5, 6, 7
Annotation description:	The paper characterizes the GAT1 knockout mice. Figure 1D demonstrates a loss of 3h-GABA uptake in hippocampal syanptosomes from the deficient mice.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:	Whole-cell patch clamp Transmembrane transport assay
Annotator(s):	Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534)
Lab:	Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
Additional literature:	The paper characterizes the first GABA transporter, named GAT1. Figure 1 demonstrates the 3H-GABA uptake assay in Xenopus oocytes injected with the GAT1 mRNA. 1A - a histogram comparing the uptake of oocytes expressing the transporter in comparison to controls. 1B - transport kinetics evaluated by increasing the 3H-GABA concentration. 1C - uptake in different buffers. 1D - Effect of GABA uptake blockers on the GABA transport. Taking together, the data points to a role of the GAT1 protein in GABA transport. @ PMID:1975955
SynGO annotation ID:	762
Dataset release (version):	20231201
View annotation as GO-CAM model: