| Annotated protein: | Sodium- and chloride-dependent creatine transporter 1 (CHOT1) (CT1) (Creatine transporter 1) (Solute carrier family 6 member 8). Gene symbol: SLC6A8. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P28570 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A8 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Dodd JR, et al. "Functional and immunocytochemical characterization of the creatine transporter in rat hippocampal neurons" J Neurochem. 2010 Nov;115(3):684-93 PMID:20731764 |
| Figure(s): | Fig.3 and 4 |
| Annotation description: | The creatine transporter is a plasma membrane protein localized to the soma and neuronal processes of cultured rat hippocampal neurons (Fig. 3,4). Colabelling with MAP2 reveals a dendritic localization (Fig.3) and CRT co-localizes with the NR1 subunit of NMDA receptors (Fig.4) indicating a postsynaptic localization. CRT antibody was produced in rabbits and affinity-purified as described previously (Mak et al. 2009 - reference 2): "Briefly, antibodies were prepared by immunising rabbits with a C-terminal fragment of the bovine CRT, expressed as a fusion protein with glutathione-S-transferase (GST) from the parasitic helminth, Schistosoma japonicum. GST-reactive antibodies were removed on a column containing immobilised GST. Antibodies, specific for the C-terminal 21 amino acids of the CRT were affinity-purified on a column containing immobilised GST fusion protein (GST-CC2) and stored (0.5 mg/mL) in phosphate buffer (-80 °C). The affinity-purified CRT antibodies recognise a ~70 kDa glycoprotein in membranes from human embryonic kidney (HEK) 293 cells, transiently or stably expressing the CRT and also showed strong staining of the plasma membrane of these cells by immunofluorescence microscopy." No KO control. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Description of the proposed membrane topology of the creatine transporter. @ PMID:18652074 Immunohistochemistry of rat brain reveals creatine transporter expression in neurons. Description of the used antibody. @ PMID:19580854 |
| SynGO annotation ID: | 774 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |