| Annotated protein: | Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]. Gene symbol: ACTB. Taxonomy: Homo sapiens (Human). Uniprot ID: P60709 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTB |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynaptic actin cytoskeleton (GO:0098973) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Honkura N, et al. "The subspine organization of actin fibers regulates the structure and plasticity of dendritic spines" Neuron. 2008 Mar 13;57(5):719-29 PMID:18341992 |
| Figure(s): | Fig 1,2, 4 |
| Annotation description: | The authors use a fluorescently labeled overexpression of human b-actin to study the dynamics (G-actin/F-actin transitions) of the actin cytoskeleton in dendritic spines. As inferred by the curator and based on the author's intent it is derived that the b-actin gene provides the structural basis of the F-actin skeleton at the postsynapse. The "structural component" term is derived from Fig.4 where local application of the actin polymerization inhibitor Latrunculi A resulted in reduction of actin distribution in the synapse and the retraction of the spine. For this reason this term is not annotated against a IC evidence code. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Optical physiology |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 812 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |