| Annotated protein: | Sodium-dependent serotonin transporter (SERT) (5HT transporter) (5HTT) (Solute carrier family 6 member 4). Gene symbol: SLC6A4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P31652 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | striatum |
| Annotated paper: | Pickel VM, et al. "Ultrastructural localization of the serotonin transporter in limbic and motor compartments of the nucleus accumbens" J Neurosci. 1999 Sep 1;19(17):7356-66 PMID:10460242 |
| Figure(s): | Fig. 1, 5, 6 |
| Annotation description: | SERT immunoreactivity can be found on the plasma membrane in some dendrites and spines of neurons that are not 5-HT positive at the ultrastructural level. Pre-embedding peroxidase labelling (Fig. 1) and immunogold-silver labelling of SERT was performed in the Nucleus accumbens (Nac). Immunogold-labelling demonstrates the presence of SERT on some dendrites and spines (Fig. 5, 6). Antibodies: antipeptide (C611-630 and E387-401) and anti-fusion protein antisera (N1-85). In control experiments, samples were processed for immunoperoxidase or immunogold labelling with the omission of primary antisera or with antisera that have been pre-absorbed with the peptide. No KO-control. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 821 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |