| Annotated protein: | Sodium-dependent dopamine transporter (DA transporter) (DAT) (Solute carrier family 6 member 3). Gene symbol: SLC6A3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61327 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A3 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter uptake (GO:0001504) |
| Synapse type(s): | striatum |
| Annotated paper: | Giros B, et al. "Hyperlocomotion and indifference to cocaine and amphetamine in mice lacking the dopamine transporter" Nature. 1996 Feb 15;379(6566):606-12 PMID:8628395 |
| Figure(s): | Fig. 1d, Fig. 5 |
| Annotation description: | The plasma membrane dopamine transporter (DAT) transports dopamine into neurons. Striatal synaptosomes from DAT-KO mice show no uptake of radiolabelled dopamine (Fig. 1d). Fast-scan cyclic voltammetry (carbon-fibre amperometry) after electrical stimulation in striatal DAT-KO slices reveals a 100times longer time for dopamine clearance in comparison to wildtype slices (Fig. 5). These findings indicate a defect in dopamine re-uptake in DAT-KO mice. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) Transmembrane transport assay |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 837 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |