| Annotated protein: | Calcium/calmodulin-dependent protein kinase type II subunit alpha (CaM kinase II subunit alpha) (CaMK-II subunit alpha) (EC 2.7.11.17). Gene symbol: CAMK2A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P11275 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAMK2A |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | forebrain |
| Annotated paper: | Swulius MT, et al. "Structure and composition of the postsynaptic density during development" J Comp Neurol. 2010 Oct 15;518(20):4243-60 PMID:20878786 |
| Figure(s): | Figure 8, Table 2, Table 1 |
| Annotation description: | The paper characterizes the localization of Calcium /Calmodulin kinase II beta and alpha in isolated post-synaptic density fractions. Figure 8 presents a quantification of the immunogold labeling as a function of age. Substantial labeling densities are present for both isoforms. Table 2 presents the summary of the immunogold labeling quantifications. Figure 1 presents the antibodies used. For all experiments, a negative control was made in the absence of primary antibody. The small amount of non-specific labeling was quantified and subtracted as background. Figure S1 presents western blots for each of the antibodies. A single band is seen for the CaMKII alpha and beta, however, the blots are cropped so specificity evaluation is difficult. 10/8/2017 Pim - The isolated also contain NMDAR subunits so in ference to the cytoplasmic part of the PSD is not valid. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 889 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |