| Annotated protein: | Erbb2 interacting protein. Gene symbol: ERBIN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: M0R9T2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ERBIN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization (GO:0099572) |
| Synapse type(s): | brain, glutamatergic Neuro-muscular junction |
| Annotated paper: | Huang YZ, et al. "Erbin is a protein concentrated at postsynaptic membranes that interacts with PSD-95" J Biol Chem. 2001 Jun 1;276(22):19318-26 PMID:11279080 |
| Figure(s): | Fig 5A & B. Fig 7B, Fig 8B, Fig 9A |
| Annotation description: | Fig 5A: Stringent washes (i.e. high concentration of salt, high Ph, or various detergents) of synaptic plasma membranes show that erbin is tightly associated with membrane fractions. Fig 5B: Erbin was not expressed as a surface protein as indicated by immunoblots of pull-downs of HEK 293T cells (expressing endogenous erbin) that were surface-biotinylated and lysed. Fig 7B: Neuromuscular Junctions expressing Erbin (Fig. 6B) at muscles that were denervated, and where the presynaptic input degenerated, kept expressing Erbin. This validates the annotation of a postsynaptic expression of Erbin at Neuromuscular Junctions. Fig 8B: Rat brain homogenates were subjected to sequential centrifugations to yield cytosol and synaptosomes. Washed synaptosomes were fractionated further to generate synaptosomal plasma membrane, which was treated with Triton X-100 to yield the postsynaptic density enriched with Erbin (in Western Blots). Fig 9A. PSD-95 was detected in Erbin immunocomplexes of deoxylate-solubilized synaptosomes, indicating an interaction between PSD-95 and Erbin in rat brain. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| SynGO annotation ID: | 893 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |