Annotated protein:Neurexin-1 (Neurexin I-alpha) (Neurexin-1-alpha). Gene symbol: NRXN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9CS84
antibody wiki:
SynGO gene info:SynGO data @ NRXN1
Ontology domain:Cellular Component
SynGO term:integral component of presynaptic membrane (GO:0099056)
Synapse type(s):hippocampus, glutamatergic
Schaffer collateral synapse (CA3->CA1)
Annotated paper:Fairless R, et al. "Polarized targeting of neurexins to synapses is regulated by their C-terminal sequences" J Neurosci. 2008 Nov 26;28(48):12969-81 PMID:19036990
Annotation description:The authors investigated a transgenic mouse line expressing Neurexin 1-alpha with an extracellular HRP tag. Functionality of this transgene was shown by rescue of Neurexin triple KO phenotypes in Zhang et al. J Neurosci. 2005, 25:4330-42. PMID: 15858059

Fig. 4 shows that Neurexin 1-alpha labeling was observed mainly in the ER and at presynaptic terminals. Labeling included the presynaptic plasma membrane but was also present at presynaptic vesicular structures.

22/6/2017 Pim
- The "intergral component of ..." part of the selected term was derived from the transmembrane domain(s) of the protein.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Annotator(s):Tony Cijsouw (ORCID:0000-0003-0912-1514)
Thomas Biederer (ORCID:0000-0002-3670-7863)
Lab:Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA
Additional literature:This paper shows in young mice, using immunoEM with a pan-neurexin antibody against all isoforms, that neurexins exhibit presynaptic localization, and also were found at postsynaptic sites. This approach did not allow to distinguish between Neurexin isoforms.
Note that Fairless et al. (2008) PMID 19036990 did not observe a postsynaptic localization for Neurexin 1-alpha. @ PMID:17360903
SynGO annotation ID:894
Dataset release (version):20210225
View annotation as GO-CAM model:Gene Ontology