| Annotated protein: | Contactin-6 (Neural recognition molecule NB-3) (mNB-3). Gene symbol: CNTN6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JMB8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNTN6 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of presynaptic membrane (GO:0099026) |
| Synapse type(s): | Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Sakurai K, et al. "Contribution of the neural cell recognition molecule NB-3 to synapse formation between parallel fibers and Purkinje cells in mouse" Dev Neurobiol. 2009 Oct;69(12):811-24 PMID:19672956 |
| Figure(s): | Fig. 3 |
| Annotation description: | Analyzing parallel fiber inputs to Purkinje cells, Fig.3A shows that most vGLUT1 puncta overlapped with the punctate contactin-6 staining. Dense contactin-6 puncta were apposed to dendritic mGluR1a of Purkinje cells. These data support a presynaptic localization of contactin-6. 21/6/2017 Pim - The rationale is: (1) protein expressed in axons (parallel fibers). (2) colocalization with pre- and postsynaptic marker proteins. (3) localization in synaptosomal preparations. - Although superresolution data is not presented, based on cell type expression and colocalization data the authors conclude: "Col-lectively, these results indicate that NB-3 is localized presynaptically at glutamatergic synapses." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Hydrophobicity analysis (Kyte and Doolittle, 1982) revealed that hNB-3 has the typical features observed in GPI-anchored membrane proteins @ PMID:9486763 The authors show that endogenous NB-3 protein in cultured cortical neurons from wild-type is expressed at the surface, a disrupted GPI consensus site in NB-3 strongly reduces membrane expression in COS cells. The authors do show that PTPalpha is able to stabilize NB-3 at the plasma membrane independent of the GPI link. @ PMID:21622556 |
| SynGO annotation ID: | 953 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |