Annotated protein:	Casein kinase II subunit beta (CK II beta). Gene symbol: CKIIBETA. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: P08182
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SynGO gene info:	SynGO data @ CSNK2B
Ontology domain:	Biological Process
SynGO term:	regulation of synapse organization (GO:0050807)
Synapse type(s):	Neuro-muscular junction
Annotated paper:	Bulat V, et al. "Presynaptic CK2 promotes synapse organization and stability by targeting Ankyrin2" J Cell Biol. 2014 Jan 6;204(1):77-94 PMID:24395637
Figure(s):	Figure 1, 2, 3
Annotation description:	The authors use the drosophila NMJ to study genes involved in the stable formation of synapses. Using an RNAi screen they identify CK2b as essential for synapse formation. -Knockdown of CK2b let to the formation of postsynaptic structures (marked with dGluRIII) that are not opposed to presynaptic terminals (marked with Brp). A similar phenotype was observed in an CK2b hypomorph (CK2b-P1) and an near-complete knockout (CK2b-P1/CK2b 26-2). (Figure 1) -Only presynaptic CK2b expression is required, since knockdown in the neuron, but not in the muscle, induces the phenotype (figure 2). Additionally, the phenotype can be rescued by presynaptic, but not postsynaptic re-expression of CK2 (Figure 3). -The knockdown efficacy is demonstrated via western blot (figure 2K). Fly CK2b has 88% homology with mouse CK2b (main text) 24/11/2017 Pim - I have selected the 'regulation of ...' variant since CK2 is a signaling protein.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958)
Lab:	Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
SynGO annotation ID:	968
Dataset release (version):	20231201
View annotation as GO-CAM model: